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Saturday, July 16, 2022

METHOD DEVELOPMENT FOR ESTIMATION OF PIPERINE IN POLYHERBAL LOZENGES BY UV SPECTROSCOPIC METHOD.

ABSTRACT

Polyherbal lozenges are immensely popular in today’s world, due to its highly palatable taste, cheap rate, soothing effect, counter-irritant effect, decreasing abdominal flatulence, and for treatment of anorexia. Herbs of Piper species are one of the most important ingredients of polyherbal lozenges and Piperine is the main chemical constituent of Piper species. In the present study an attempt was made for the estimation of piperine in ayurvedic lozenges sample by using UV-spectrophotometer. Three samples of lozenges from different brands have been selected for this purpose. The amount of piperine in each lozenge of all the three samples (S-1, S-2, and S-3) was found to be 3.1 μg, 2.7 μg and 2.8 μg respectively. Recovery studies were carried out by standard addition method and the average % recovery of the three samples S-1, S-2, S-3 were found to be 98.62%, 99.32%, and 98.35% respectively.

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