ABSTRACT
Calendula officinalis is a plant that possess many pharmacological actions like Stomach upset, Ulcers, Menstural problems, Eye infections, Inflamations, Wound healing, Antiseptics, most widely it is used as if the leaves of that plant rubes on the affected part where the bleeding occurs, it reliefes from pain & immediately stop bleeding. In our current study, we first tried to evaluate out the active ingredients present in that leave that responsible for this action by the help of some phytochemical screening & Thin Layer Chromatographic study. Then we made 2 different extracts of that leave, first with Butanol & the other in water. Then we perform the Prothrombin Time (PT) Test of these two extracts by the help of brain tissue thromboplastin as standard. And after that we also perform the quantitative study on these two extracts of that leave. In the result of PT test we found that when we treated butanol extract with citrated plasma, the coagulation occurs at 4 min 10 sec. when water extract treated with citrated plasma the coagulation occurs at 1 min. & when we treated water extract & citrated plasma with CaCl2 the coagulation occurs at 2 min. After that when we treated the water extract & citrated plasma with brain tissue thromboplastin the coagulation occurs at 20 sec. & then Butanol extract & citrated plasma with brain tissue thromboplastin the coagulation occurs at 2 min. So after performing this study we can come to a conclusion that the water extract of the Calendula officinalis leaves has the more coagulating activity.
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